FIG. 2.

Representative macroscopic images (A) showing a native, fresh intact porcine aortic root in top view (1) and a fresh root cut open with one cusp removed to reveal the main internal anatomical coordinates (2), a fully decellularized aortic root (16-day perfusion) in top view (3) and cut open (4). (B) Representative histology images of fresh aortic root sections stained for nuclei with DAPI (blue) and overlaid with elastin autofluorescence (green) before (native) and after immersion decellularization (Imm) and after 8 days (D8) and 16 days (D16) of perfusion decellularization. White arrows point to DAPI-stained cell remnants. DAPI, 4′,6-diamidino-2-phenylindole. Color images available online at www.liebertpub.com/tec