Table 4. Mutations identified from the DNA sequencing of INH and RIF resistance associated loci.
| Gene (Number of Isolates Screened) | Mutation | Effect of Mutation | Number of isolates with specific SNP |
|---|---|---|---|
| inhApro (51) | −8T/C | - | 2 (3.9%) |
| −15C/T | - | 4 (7.8%) | |
| −47G/C | - | 5 (9.8%) | |
| −102G/C | - | 5 (9.8%) | |
|
|
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| katG (51) | G944C & C723G | S315T & P241P | 1 (1.9%) |
| G944C | S315T | 39 (76.5%) | |
| G944A, A949G & C723G | S315N, I317V & P241P | 1 (1.9%) | |
| G(C)944(5)C(T) | S315T | 1 (1.9%) | |
| G944C & C1132T | S315T & L378L | 1 (1.9%) | |
|
|
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| rpoB (16) | C1163T & C1349T | S388L* & S450L | 1 (6.3%) |
| A1226G & C1349T | Q409R@ & S450L | 1 (6.3%) | |
| C1349T | S450L | 5 (31.3%) | |
| C1333T | H445Y | 5 (31.3%) | |
| C1333G | H445D | 2 (12.5%) | |
| A1334G | H445R | 1 (6.3%) | |
| A1304T | D435V | 1 (6.3%) | |
NB: The reference gene (rpoB) used here is the MTBC (H37Rv) and not the E. coli variant.
*
-compensatory rpoB mutation identified by whole genome sequencing of rifampicin resistant M. tuberculosis isolates (Comas et al., 2012).
@
- a novel mutation which may be a compensatory rpoB mutation.