Fig. 3.

Frequency of on-target and off-target Cas9 activity on the HvPM19 gene copies at T₁. a Alignment of sgRNAHvPM19-1 and sgRNAHvPM19-3 target sequences (grey highlight) with the corresponding sequences of the other copies of HvPM19. Hyphens represent alignment matches while mismatches are shown in black highlight and white font. The PAM is highlighted in red and the numbering of nucleotides is relative to the PAM. b Percentage of T₁ plants with mutations in the corresponding copies of HvPM19 for sgRNAHvPM19-1 (T₀-181 and T₀-122) and sgRNAHvPM19-3 (T₀-211). Dark and light grey bars represent the percentages for HvPM19-1 and HvPM19-3 editing, respectively