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. 2015 Nov 30;16:258. doi: 10.1186/s13059-015-0826-7

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© Lawrenson et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Frequency of on- and off-target Cas9 activity in L2F1_8.2 T₁ Brassica plants. a The alignment of sgRNA1BolC.GA4.a and sgRNA2BolC.GA4.a target sequences in BolC.GA4.a with their corresponding sequences in BolC.GA4.b. Hyphens represent alignment matches while mismatches are shown in black highlight and white font. The PAM is highlighted in red and numbering of nucleotides is relative to the PAM. b Percentage of the T₁ plants with mutations in BolC.GA4.a and BolC.GA4.b. Dark and light grey bars represent the percentages of BolC.GA4.a and BolC.GA4.b editing, respectively. N = 90 plants