Table 1.
Summary of transgenerational RNA-guided Cas9 activity and segregation in barley
| T-DNA Construct | T0 line | T1 mutation type | T1 line | Number of T2 plants screened for T-DNA | Plants without T-DNA | Plants without T-DNA and with (homozygous/heterozygous) mutationsa |
|---|---|---|---|---|---|---|
| pPM19-1 | T0-181 | Homozygous | T1-181_B5 | 4 | 0 | - |
| T1-181_E1 | 12 | 5 | 5/0 | |||
| Heterozygous | T1-181_A11 | 12 | 3 | 1/1 | ||
| T1-181_B1 | 8 | 2 | 0/0 | |||
| T1-181_B8 | 11 | 0 | - | |||
| T1-181_C1 | 9 | 0 | - | |||
| T1-181_C12 | 1 | 0 | - | |||
| T1-181_C3 | 12 | 0 | - | |||
| T1-181_C4 | 12 | 1 | 0/0 | |||
| T1-181_C9 | 11 | 1 | 0/1 | |||
| T1-181_D11 | 12 | 4 | 1/1 | |||
| T1-181_D2 | 12 | 3 | 2/0 | |||
| T1-181_D9 | 2 | 0 | - | |||
| T1-181_E4 | 12 | 0 | - | |||
| T1-181_G4 | 11 | 3 | 0/1 | |||
| T1-181_G5 | 12 | 1 | 0/0 | |||
| T1-181_H2 | 12 | 3 | 1/1 | |||
| T1-181_H5 | 12 | 0 | - | |||
| T1-181_H9 | 12 | 4 | 0/0 | |||
| T0-122 | Heterozygous | T1-122_B11 | 12 | 4 | 2/1 | |
| T1-122_C6 | 12 | 1 | 0/1 | |||
| T1-122_F12 | 12 | 4 | 0/3 | |||
| T1-122_H2 | 12 | 0 | - | |||
| T1-122_H4 | 12 | 3 | 0/0 | |||
| T1-122_H9 | 12 | 0 | - | |||
| pPM19-3 | T0-211 | Heterozygous | T1-211_B11 | 12 | 3 | 0/0 |
| T1-211_D10 | 12 | 0 | - | |||
| T1-211_G4 | 7 | 0 | - |
aHyphens (-) indicate that all plants had presence of the T-DNA construct, and thus were not tested