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. Author manuscript; available in PMC: 2015 Nov 30.
Published in final edited form as: Methods Cell Biol. 2015 Jun 11;130:101–126. doi: 10.1016/bs.mcb.2015.03.020

Figure 2.

Figure 2

Calculation of Arf1 nucleotide exchange kinetics
  1. Unprocessed tryptophan fluorescence of Sec7(203-1220)-mediated exchange of Arf1 nucleotide in triplicate. Components are sequentially added as indicated; Sec7 concentration is proportional to the indicated difference in fluorescence.
  2. Arf1 nucleotide exchanges from (a) extracted and zeroed to baseline.
  3. One exchange reaction from (b) fit to a single exponential with linear drift component.
  4. Exchange reactions from (b) normalized for publication via curve fitting as per (c).