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Demonstration of interaction of Sec7 and Arf1 by liposome flotation
Liposomes of TGN composition are incubated with Arf1 possessing or lacking its membrane interaction domain and binding either GDP (inactive) or GMPPNP (GTP*, active). Sec7(203-2009) is added and flotation performed, followed by SDS-PAGE and Coomassie staining.
