Table 3.
Construct | MW (kDa) | A280 at 1 mg/ml * |
---|---|---|
6His-TEV-Sec7 203-2009 | 208.8 | .731 |
6His-TEV-Sec7 203-1220 | 116.6 | .530 |
6His-TEV-Sec7 203-1017 | 93.8 | .462 |
myrArf1 | 20.5 | 2.14 |
6His-TEV-ΔN17Arf1 | 21.1 | 2.16 |
ΔN17Arf1 | 18.8 | 2.34 |
myrArl1 | 20.4 | 2.15 |
6His-TEV-ΔN18Arl1 | 20.8 | 1.92 |
ΔN18Arl1 | 18.5 | 2.08 |
A280 is estimated for the primary sequence using ProtParam (Gasteiger et al., 2005), with absorbance of a single guanine nucleotide (9000 M−1 cm−1) (Sontag, Sage, & Erickson, 2009) added for the small GTPases.