. Author manuscript; available in PMC: 2015 Nov 30.

Published in final edited form as: Methods Cell Biol. 2015 Jun 11;130:101–126. doi: 10.1016/bs.mcb.2015.03.020

Table 3.

Physical properties of protein constructs

Construct	MW (kDa)	A₂₈₀ at 1 mg/ml ^*
6His-TEV-Sec7 203-2009	208.8	.731
6His-TEV-Sec7 203-1220	116.6	.530
6His-TEV-Sec7 203-1017	93.8	.462
myrArf1	20.5	2.14
6His-TEV-ΔN17Arf1	21.1	2.16
ΔN17Arf1	18.8	2.34
myrArl1	20.4	2.15
6His-TEV-ΔN18Arl1	20.8	1.92
ΔN18Arl1	18.5	2.08

A₂₈₀ is estimated for the primary sequence using ProtParam (Gasteiger et al., 2005), with absorbance of a single guanine nucleotide (9000 M⁻¹ cm⁻¹) (Sontag, Sage, & Erickson, 2009) added for the small GTPases.