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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Cell Physiol. 2016 Mar;231(3):630–640. doi: 10.1002/jcp.25108

Figure 1. The cloudy eyed mutation of MITF leads to denser bone in neonatal mice due to defective osteoclastogenesis.

Figure 1

A. Schematic representation of the MITF protein with the location of the Mitfce mutation indicated. The region deleted with the Mitfce mutation is underlined with the red bar.

B. Representative longitudinal and metaphyseal cross section images from microCT analysis of the femurs of four day old mice homozygous for the cloudy eyed mutation of MITF (Mitfce/ce) or heterozygous controls (Mitfce/+)

C. Quantification of bone volume to total volume (BV/TV) and trabecular thickness (Tb.Th) from microCT analysis of four day old Mitfce/ce mice and controls (Mitfce/+), n = 3. Data are represented as the mean +/− S.D.

D. Representative images of TRAP staining to mark mature osteoclasts in the distal femurs of four day old Mitfce/ce mice and Mitfce/+ controls. Scale bars = 100μm.

E. Histomorphometric quantification of osteoclast surface area to trabecular surface area (Oc.S/BS) and osteoclast number per trabecular perimeter (Oc.No/Tb.pm) from TRAP staining shown in C, n = 3. Data are represented as the mean +/− S.D.