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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: Chem Res Toxicol. 2015 Jul 16;28(8):1584–1594. doi: 10.1021/acs.chemrestox.5b00157

Figure 6. LicA down-regulated TCDD-induced P450 1B1 mRNA expression, P450 1A/1B activity, and XRE-luciferase activity.

Figure 6

Figure 6

A) P450 1B1 mRNA expression was analyzed via qPCR after 24 h treatment of MCF-10A cells with TCDD (10 nM) and licorice compounds (10 μM). B) P450 1A/1B activity measured via EROD assay in MCF-10A cells after treatment with compounds (10 μM) 1 h prior to treatment with TCDD (10 nM) for an additional 48 h. C) HepG2 cells were incubated with TCDD (10 nM) and LicA for 24 h before analysis of XRE-luciferase reporter activity. Results are the means ± SEM of three independent experiments analyzed by one-way ANOVA with Dunnett’s multiple comparison post-test, *p < 0.001.