Table 2.

P. furiosus strains used in this study

P. furiosus strain	Parent strain	Expression construct for M. sedula gene	Genetic marker	Reference
COM1	-	None	NONE	(Lipscomb et al., 2011)
MW56	COM1	Pslp-accABC-mcr-msr	Pgdh-pyrF	(Keller et al., 2013)
MW76	COM1	Pslp-accABC-mcr-msr-bpl-ca	Pgdh-pyrF	This work

(see Table 1 for enzyme abbreviations)

P_gdh – P. furiosus glutamate dehydrogenase gene promoter

P_slp – P. furiosus S-layer protein gene promoter

P_pep – P. furiosus PEP synthase gene promoter