Table 6.
Summary of antibody dilutions and conditions used in immunohistochemistry/immunofluorescence analysis of mitochondrial dynamics and synaptic proteins in WT and mutant Htt neurons
| Markers | Primary antibody: species and dilution | Purchased from company, city and state | Secondary antibody, dilution, Alexa dye | Purchased from company, city and state |
|---|---|---|---|---|
| Drp1 | Rabbit Polyclonal 1:300 | Novus Biological, Littleton, CO | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 |
KPL, Gaithersburg, MD VECTOR Laboratories, Inc. Burlingame, CA Molecular Probes, Grand Island, NY |
| Fis1 | Rabbit Polyclonal 1:300 | Protein Tech Group, Inc., Chicago, IL | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 |
KPL, Gaithersburg, MD VECTOR Laboratories, Inc. Burlingame, CA Molecular Probes, Grand Island, NY |
| Mfn1 | Rabbit Polyclonal 1:300 | Abcam, Cambridge, MA | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1: 200), TSA-Alexa594 |
KPL, Gaithersburg, MD VECTOR Laboratories, Inc. Burlingame, CA Molecular Probes, Grand Island, NY |
| CypD | Mouse Monoclonal 1:500 | EMD, Calobiochem Chemicals, Inc., Gibbstown, NJ | Goat anti-mouse Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories, Inc., Burlingame, CA Molecular Probes, Grand Island, NY |
| Cyt B | Rabbit Polyclonal 1:500 | Santa Cruz Biotechnology, Inc., Dallas, TX | Goat anti-mouse Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories, Inc., Burlingame, CA Molecular Probe, Grand Island, NY |
| SYN | Rabbit Polyclonal 1:400 | Thermo Fisher Scientific, Inc., Rockford, IL | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories, Inc., Burlingame, CA Molecular Probes, Grand Island, NY |
| PSD95 | Rabbit Monoclonal 1:300 | Abcam, Cambridge, MA | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa594 | KPL, Gaithersburg, MD VECTOR Laboratories, Inc., Burlingame, CA Molecular Probes, Grand Island, NY |
| MAP2 | Rabbit Polyclonal 1:600 | Santa Cruz Biotechnology, Inc., Dallas, TX | Goat anti-rabbit Biotin 1:400, HRP-Streptavidin (1:200), TSA-Alexa488 | KPL, Gaithersburg, MD VECTOR Laboratories, Inc., Burlingame, CA Molecular Probe, Grand Island, NY |