Table 1.
Changes in lipid species in SOD1(G86R) mice
| Stage | Spinal cord |
Muscle |
||
|---|---|---|---|---|
| A | ||||
| Pre-symptomatic | 78 | 48% | 76 | 26% |
| Pre-symptomatic and symptomatic | 37 | 23% | 69 | 24% |
| Symptomatic | 46 | 29% | 147 | 50% |
| Lipid species with significant changes | 161 | 292 | ||
| Detected lipid species | 1599 | 1518 | ||
| Stage | Regulation | Spinal cord |
Muscle |
||
|---|---|---|---|---|---|
| B | |||||
| Pre-symptomatic | Down | 96 | 84% | 8 | 6% |
| Up | 19 | 16% | 137 | 94% | |
| Σ | 115 | 145 | |||
| Symptomatic | Down | 64 | 77% | 81 | 38% |
| Up | 19 | 23% | 135 | 62% | |
| Σ | 83 | 216 | |||
Specific changes between SOD1(G86R) and WT mice were identified using a multivariate PLS-DA model. A SOD1(G86R)-to-WT ratio was considered significant when the corresponding correlation coefficient in the S-plot built after the model was less than or equal to −0.7 (for down-regulated species) or greater than or equal to +0.7 (for up-regulated species). A shows the number of total changes observed in pre-symptomatic and symptomatic mice whereas B shows their classification into down- and up-regulated subsets (see Supplementary Fig. 1 and Statistical Analysis section for more details).