Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cell Microbiol. 2014 Nov 22;17(4):504–519. doi: 10.1111/cmi.12380

Fig. 5.

Fig. 5

Localization of SUMO1 and AmpA in A. phagocytophilum infected mammalian host cells. A. Representative images of infected RF/6A cells at 12, 16, 20, 24, and 28 h post infection. Time points are marked at the left of each row. Cells were immunofluorescently stained to detect AmpA (green), SUMO1 (red), and DNA (DAPI, blue). Merged images are shown in the third column. Areas demarcated by white hatched boxes in the merged column are magnified five-fold in the fourth column. White arrows indicate representative areas where AmpA and SUMO1 signals colocalize. Scale bars, 20 μm. B. SUMO1 (red) and DNA (DAPI, blue) staining in an uninfected RF/6A cell. C and D. Merged confocal images of RF/6A cells infected with a low MOI of A. phagocytophilum (C) and A. phagocytophilum infected HL-60 cells (D) stained for AmpA (green), SUMO1 (red), and DNA (DAPI, blue) at 24 h post infection.