Figure 3.

Intracerebroventricular (ICV) injection of TCA and GCDA into normal rats suppresses the HPA axis. Rats were treated with a single injection of the bile acids CA, DCA, CDCA, TCA, and GCDA (20 pmol) into the third ventricle. After 6 hours, serum corticosterone levels were assessed by EIA (A), relative CRH mRNA expression in the hypothalamus of TCA or GCDA-treated rats was assessed by real-time PCR (B), and serum concentrations of CRH were assessed in TCA or GCDA-treated rats by EIA (C). *, P < .05 compared with vehicle (n = 4).