Fig 1. IFN-I signaling is essential to induce the rapid and concerted recruitment of Ly-6C^hi monocytes and CD11c⁺ DCs in mucosal and draining LN tissues.

(A) Leukocyte infiltration of vaginal tract and iliac LN in infected BL/6 or IFNAR KO mice. (B,C) Accumulated number of infiltrated leukocyte subsets. Cells were prepared from vaginal tract (B; VT) and iliac LN (C; ILN) by collagenase digestion at 12, 24, 48, and 72 h pi and subcellular proportions of CD11b⁺Ly-6C^hi and CD11b⁺Ly-6G^hi leukocytes were determined using flow cytometric analysis. Values in the representative dot-plots denote the average percentages of each population derived from at least four independent samples after gating on CD11b⁺ cells. Data in the bar chart represent the average ± SD of values derived from three individual experiments (n = 4–5). *, p<0.05; **, p<0.01; ***, p<0.001 compared with the levels of BL/6 or IFNAR KO mice at the indicated time point.