Fig 3. Small Molecule and Glycosylation Effects on GAPDH Stability in Arctica islandica.

Lysates from Arctica islandica were filtered through a 30kDa centricon, isolating GAPDH from any potential small stabilizers. Another sample was treated with a deglycosylation kit to remove N-linked and O-linked carbohydrate modifications. These depleted lysates were pre-stressed for twenty minutes in 3.5 M urea. Glyceraldehyde 3-phosphate was then added, and the activity of endogenous GAPDH was monitored as ΔA₃₄₀/minute, corresponding to the reduction of NAD⁺ to NADH. Data is reported as the fold change from unstressed activity. GAPDH stability was not further compromised by deglycosylation (p = 0.62) or the removal of small molecules (p = 0.85) as compared to the unmodified control.