Fig 3. G-quadruplex regulation of Luciferase gene expression.
(A) mRNA levels as assessed by qPCR and represented ΔΔCT values represents the normalization of the ΔCT of firefly luciferase to the ΔCT renilla luciferase then normalized to the wild type. (B) Quantification of Dual Luciferase Assay comparing the wild type G-quadruplex sequence to the mutant G-quadruplex sequence (G-quad Mut). Empty Firefly Luciferase plasmid was used as a control which does not contain the 3’UTR of APP. Firefly Luciferase values were normalized to Renilla Luciferase.