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. 2015 Nov 30;10(11):e0143974. doi: 10.1371/journal.pone.0143974

Fig 2. Gating for round and irregular shapes of Blastocystis.

Fig 2

The shapes of Blastocystis were selected based on aspect ratios from the brightfield channel (M03) and CFSE staining (M02). Viable and non-viable cells were plotted separately. The above graphs shows the gating for Blastocystis ST1-NUH9 isolate (A). The average proportion of round and irregular shapes fond in cultures of Blastocystis ST1-NUH9, ST4-WR1 and ST7-B were then plotted in a graph (B). These are based on three separate batches of cultures and independent runs in ImageStream. Error bars signify standard error values. p-values comparing the proportion of round cells between viable and non-viable populations are 0.06, 0.47 and 0.31 for ST1-NUH9, ST4-WR1 and ST7-B isolates, respectively.