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. 2015 Nov 30;10(11):e0143974. doi: 10.1371/journal.pone.0143974

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© 2015 Yason, Tan

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Blastocystis cells were plotted according to circularity using the software’s shape wizard. The population of cells was then divided into three groups: low, mid and high circularity (A). A dot-plot was generated to analyze the relationship between circularity and DNA content indicated by Hoechst staining of Blastocystis cells (B). The dot-plot shows that some highly circular cells have the highest DNA content while the least circular cells have the lowest DNA content. Bar graphs show the average Hoechst-staining of the three groups of Blastocystis cells based on circularity (C). Differences between groups based on circularity in each ST were found to be significant (p < 0.05) using ANOVA.