Fig. 3.
An inter-SH3 linker in Nck promotes actin network assembly by N-WASP. (A) Experimental strategy for localization of Nck to membranes containing pY3-nephrin. (B) Nck constructs used in the deletion analysis. (C) Representative images of actin tails formed by Nck deletion constructs. Motility reactions contained 200 nM N-WASP and 100 nM Nck. (Scale bar: 5 μm.) (D) Integrated fluorescence intensity of Alexa488 actin tails. (E) Actin tail length. (F) Integrated fluorescence intensity of bead-localized Alexa568 Nck constructs. (Mean ± SD; n > 20; ***P < 0.001.) fl., Full length; PRR, proline-rich region.