Fig. 1.
(A) Overview of the structure of the dynactin complex. (Top Left) Overall structural organization of dynactin based on the recent cryo-EM structure (21). (Middle and Bottom Left) Domain structure of p150Glued microtubule-binding subunit of dynactin. The CAP-Gly domain (colored purple) is part of the p150Glued. (B) SDS/PAGE cosedimentation gel of CAP-Gly with microtubules. The lanes are labeled in order as follows: M, molecular mass markers; F, free CAP-Gly standard; P, pellet obtained by ultracentrifugation of CAP-Gly/microtubule solution; S, supernatant after ultracentrifugation of the pellet. (C) Transmission electron microscopy (TEM) images of microtubules (Top Left); U-13C,15N,2H-CAP-Gly/MT NMR sample (Top Right). The Bottom images were recorded before and after 2 wk of continuous multidimensional MAS NMR experiments.