Fig. 3.

Toeprinting/primer extension analysis. Toeprinting analysis of IAPV IRES/ribosome complexes for helix P3.3 deletion mutations as depicted in Fig. 2C (A) and A6576 mutants (B). Bicistronic RNAs harboring the WT or mutant IRESs were incubated alone (−) or with salt-washed HeLa ribosomes (+) and analyzed by primer extension. The sequencing reactions of the WT IRES are shown on the left, with the position of the +1 nucleotide indicated for reference. The position of the observed toeprint is as denoted.