Fig. S7.

Schematic overview of the library preparation using in-vitro transposition. The EZ-Tn5 transposase mediates the fragmentation of double-stranded DNA and simultaneously ligates the DNA fragments with synthetic oligonucleotides including a unique DNA identity (UID) plus sample barcode plus sequencing adapters. The procedure includes (1) the assembly of a transposase complex (2), DNA fragmentation, and (3) library amplification. After purification, the library is ready for sequencing or whole-exome capture. Dark blue, genomic DNA; light blue/orange, partial sequencing primer; purple/pink, PCR primers and sequencing platform adapters; green, UID of 8-bp random oligonucleotides; gray hexagon, transposon; and gray triangles, 6-bp sample barcode.