Fig. 5.

HuR depletion down-regulates FMR1 mRNA levels and FMRP synthesis. (A) The distribution of mRNA changes after knockdown (KD) of HuR reveals a significant decrease in FMR1 levels compared with over 2,000 other mRNAs assayed (log₂ value −0.87). The red arrow indicates the bin in which FMR1 falls. (B) The distribution of protein-level changes shows a significant decrease in FMRP synthesis (log₂ value −1.37). The red arrow indicates the bin in which FMRP falls. (C) The distribution of protein synthesis changes when accounting for concomitant mRNA changes indicates that the decrease in protein synthesis is not only the result of a reduction in mRNA levels (i.e., −log₂ difference between protein and mRNA levels −0.51). The red arrow indicates the bin in which FMRP falls. All histograms were generated using data from Lebedeva et al. (42).