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Fluorescently tagged FMR1 mRNAs were visualized in primary cortical neurons by immunocytochemistry. (Top) FMR1 mRNA localization with a control 3′UTR. (Bottom) FMR1 mRNA localization with the 746C variant allele included in the 3′UTR. The constructs used in these experiments are described in Fig. 4A. (Scale bars, 10 μm.)
