Fig. 5.

The role of ferritin in O. tauri cell survival under iron limitation. Cell survival of WT and ΔFtn cells grown in Mf medium containing 1 µM Fe(III) citrate in the presence of increasing concentrations of DFOB under a light irradiance of 100 µmol⋅quanta⁻¹⋅m⁻²⋅s⁻¹. Cell abundances were determined by flow cytometry. (A) Cells were grown under 12-h/12-h light/dark conditions. (B) Cells entrained under 12-h/12-h light/dark conditions for 5 d were transferred under constant light corresponding to circadian free-running conditions of constant light. Data are shown as mean ± SD (n = 4). Significant differences in cell abundances between WT and ΔFtn cells were determined by the multiple t-test using the Holm–Sidak method, α = 0.5% (**P < 0.01; ***P < 0.001; ****P < 0.0001).