Fig. S3.

Effect of DFOB on the kinetics of ferritin iron unloading under day/night conditions. Cells grown in 1 µM ⁵⁵Fe(III) citrate were washed and resuspended in Mf medium. (A) Cells treated with 1.5 µM DFOB. (B) Control cells in Mf medium. Upper panels in A and B show autoradiography of ⁵⁵F-labeled ferritin from dawn (upper band) or dusk (lower band) on a blue native PAGE. Lower panels in A and B show quantization of ferritin band intensity normalized to the mean signal. During the night, DFOB had no effect on ferritin labeling.