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. 2015 Nov 9;112(47):14652–14657. doi: 10.1073/pnas.1506074112

Table S1.

Mass spectrometry analysis of the main iron-binding proteins presented as a Mascot report

Accession Description Score Coverage No. of proteins No. unique peptides No. of peptides No. of PSM Area No. of AAs Mass, kDa Calc. pI
350 kDa
 308798739 P-type ATPase (ISS) [O. tauri] 1,088.96 28.92 1 49 31 39 1,172 128.2 6.25
 308801239 Sor-like protein (ISS) [O. tauri] 1,002.01 42.94 1 19 14 41 9.294E7 347 37.5 7.39
 308799545 Glutamine synthetase, catalytic domain (ISS) [O. tauri] 913.21 43.33 1 38 24 50 8.668E7 690 75.4 5.83
 308811799 Peptide exporter, ABC superfamily (ISS) [O. tauri] 873.10 25.95 1 34 25 31 2.965E6 1,079 119.3 6.49
 308799207 Acetyl-CoA carboxylase (ISS) [O. tauri] 872.52 21.38 1 71 40 42 1.346E6 2,123 233.6 6.24
 308809732 Unnamed protein product [O. tauri] 846.71 28.82 1 31 20 31 1.250E7 864 95.1 6.62
 308801004 Pyruvate, phosphate dikinase, chloroplast [Precursor] (IC) [O. tauri] 818.15 31.15 2 40 25 40 1.071E7 931 100.4 5.25
 308807779 ACA11_ARATH Potential calcium-transporting ATPase 11, plasma membrane-type (ISS) [O. tauri] 796.92 31.73 1 55 29 34 3.013E6 1,062 114.1 6.01
 113170470 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [O. tauri] 752.73 46.95 1 36 19 40 3.484E7 475 52.5 6.48
 308811204 Chaperone HSP104 and related ATP-dependent Clp proteases (ISS) [O. tauri] 686.41 37.77 2 41 26 26 3.347E6 781 86.0 5.30
 308813361 Putative hsp70 (ISS) [O. tauri] 598.65 31.03 1 26 18 26 5.411E6 651 72.2 8.82
 308806445 Chloroplast inner envelope protein-related (ISS) [O. tauri] 597.57 21.55 1 33 23 25 1.153E6 1,174 127.6 6.18
 308802856 Magnesium chelatase H subunit (ISS) [O. tauri] 597.57 22.91 1 46 25 26 1.561E6 1,397 153.6 5.59
 308808950 NADP-glyceraldehyde-3-phosphate dehydrogenase (IC) [O. tauri] 578.93 50.79 2 20 15 23 6.713E6 378 40.2 8.25
 113170490 Atp1 [O. tauri] 565.70 37.99 1 25 17 19 5.579E6 508 55.1 5.76
 113170421 AtpB [O. tauri] 563.54 40.17 1 25 15 21 1.343E7 483 51.8 4.93
 308800160 Bip Luminal binding protein precursor, probable (IC) [O. tauri] 545.76 25.94 1 26 15 19 1.816E6 663 73.2 5.03
 113170436 TufA [O. tauri] 527.42 50.12 1 22 16 26 5.358E6 409 44.4 5.15
 308810280 RuBisCO subunit binding-protein alpha subunit, chloroplast [Prec (IC) [O. tauri] 500.14 28.35 1 27 12 13 3.977E6 575 61.2 5.10
 308806437 Transketolase (ISS) [O. tauri] 481.87 17.45 1 23 10 11 2.134E6 745 80.8 6.47
 308809675 Tricorn protease homolog (ISS) [O. tauri] 473.04 15.95 1 31 20 23 1.971E6 1,467 161.1 6.80
 308806706 Acetyl-CoA carboxylase (ISS) [O. tauri] 451.56 19.81 1 31 24 25 1.353E6 1,272 137.4 6.38
 308808432 Nia, nitrate reductase apoenzyme (IC) [O. tauri] 450.30 20.59 1 26 17 18 1.724E6 952 106.1 6.25
 308800570 Ggh geranylgeranyl reductase (IC) [O. tauri] 440.59 31.82 1 21 12 14 8.770E5 462 51.4 8.03
 308811769 Unnamed protein product [O. tauri] 436.99 11.91 2 34 14 14 6.838E5 1,536 171.8 4.83
150 kDa
 308811414 Ferredoxin-dependent glutamate synthase (ISS) [O. tauri] 1,994.81 50.72 1 63 57 104 7.169E7 1,382 148.1 5.83
 308806437 Transketolase (ISS) [O. tauri] 1,834.99 46.04 1 23 22 73 1.943E8 745 80.8 6.47
 308811328 Mitochondrial elongation factor (ISS) [O. tauri] 1,133.92 44.27 1 44 35 55 4.494E7 820 91.3 5.90
 308813361 Putative hsp70 (ISS) [O. tauri] 1,084.78 36.71 1 26 24 45 3.701E7 651 72.2 8.82
 308800160 Bip Luminal binding protein precursor, probable (IC) [O. tauri] 1,034.57 44.19 1 26 29 42 2.053E7 663 73.2 5.03
 308799607 Unnamed protein product [O. tauri] 907.30 32.43 1 12 11 34 4.117E7 441 46.9 4.49
 308810280 RuBisCO subunit binding-protein alpha subunit, chloroplast [Prec (IC) [O. tauri] 897.85 40.87 1 27 20 26 1.355E7 575 61.2 5.10
 308803252 Heat shock protein 70/HSP70 (ISS) [O. tauri] 874.42 39.52 1 25 21 27 1.068E7 673 72.0 5.67
 308808950 NADP-glyceraldehyde-3-phosphate dehydrogenase (IC) [O. tauri] 871.07 57.67 2 20 21 41 4.118E7 378 40.2 8.25
 308799163 Oxidoreductase (ISS) [O. tauri] 837.17 45.06 1 32 29 34 6.722E6 790 86.1 8.22
 308799545 Glutamine synthetase, catalytic domain (ISS) [O. tauri] 813.04 35.65 1 38 18 33 4.371E7 690 75.4 5.83
 308798869 Enolase (ISS) [O. tauri] 769.55 21.58 1 27 23 37 1.760E7 1,228 134.4 8.66
 308806027 Rubisco subunit binding-protein beta-2 subunit (60 kD Chaperonin (IC) [O. tauri] 758.16 37.36 1 30 22 25 1.113E7 621 65.9 5.49
 308806473 Elongation factor G, chloroplast precursor (ISS) [O. tauri] 729.53 30.63 1 23 20 26 7.540E6 790 86.5 5.44
 308810507 EF-1 alpha-like protein (ISS) [O. tauri] 726.56 45.92 3 29 21 33 2.520E7 490 54.1 8.16
100 kDa
 308798869 Enolase (ISS) [O. tauri] 1,186.95 21.58 1 27 23 51 1.848E7 1,228 134.4 8.66
 308808950 NADP-glyceraldehyde-3-phosphate dehydrogenase (IC) [O. tauri] 1,029.64 52.38 2 20 17 40 2.606E7 378 40.2 8.25
 308813361 Putative hsp70 (ISS) [O. tauri] 1,023.10 35.64 1 26 22 41 1.883E7 651 72.2 8.82
 308808434 Nii. plastid-targeted nitrite reductase apoenzyme (IC) [O. tauri] 981.43 44.22 1 43 38 53 1.062E7 986 108.4 7.08
 308806437 Transketolase (ISS) [O. tauri] 902.05 27.65 1 23 15 28 8.908E6 745 80.8 6.47
 308812292 MGC53673 protein (ISS) [O. tauri] 862.74 53.92 1 33 29 49 1.205E7 651 71.2 8.02
 113170421 AtpB [O. tauri] 848.06 44.10 1 25 16 34 1.057E7 483 51.8 4.93
 308813696 Predicted serine-pyruvate aminotransferase (ISS) [O. tauri] 814.87 24.19 1 19 14 36 2.226E7 868 93.7 7.18
 308806027 Rubisco subunit binding-protein beta-2 subunit (60 kD Chaperonin (IC) [O. tauri] 766.87 44.12 1 30 23 29 9.201E6 621 65.9 5.49
 308801693 Putative ketol-acid reductoisomerase (ISS) [O. tauri] 712.47 33.58 1 22 16 25 8.480E6 545 59.3 6.74
 308802948 RuBisCO Activase (IC) [O. tauri] 692.59 49.39 1 21 15 29 1.089E7 407 44.5 6.44
 113170436 TufA [O. tauri] 689.08 42.79 1 22 14 25 7.940E6 409 44.4 5.15
 308800160 Bip Luminal binding protein precursor, probable (IC) [O. tauri] 671.20 32.13 1 26 20 25 6.636E6 663 73.2 5.03
 308809581 Cytosolic phosphoglucose isomerase (ISS) [O. tauri] 664.07 29.32 2 18 17 29 7.081E6 590 65.0 5.86
 308806473 Elongation factor G, chloroplast precursor (ISS) [O. tauri] 653.82 30.89 1 23 20 30 4.777E6 790 86.5 5.44
 308810280 RuBisCO subunit binding-protein alpha subunit, chloroplast [Prec (IC) [O. tauri] 636.98 37.91 1 27 17 18 2.468E6 575 61.2 5.10
 308808936 Putative UV-damaged DNA binding factor (ISS) [O. tauri] 634.55 20.90 2 27 23 25 1.786E6 1,282 140.8 5.34

The main radiolabeled bands at 350, 150, 100, and 70 kDa were excised from the blue native PAGE (Fig. 4) and analyzed by mass spectrometry as described in Methods. AAs, amino acids; Calc. pl, calculated protein isoelectric point; PSM, peptide spectrum matches.