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. 2015 Nov 11;112(47):E6515–E6524. doi: 10.1073/pnas.1519925112

Fig. S4.

Fig. S4.

CS transgene regulates B-cell development independently of endogenous CD45. (A) Plots represent CD19+ splenocytes from allelic series mice (−/−, L/−, or +/− endogenous CD45 alleles) in the absence or presence of a single copy of the CS Tg stained with CD21 and CD23 to identify MZ B cells (CD21hi CD23lo). (B and D) histograms represent MZ B cells (B) as gated above or CD23+ splenic B cells (D) from allelic series mice with (blue) or without (red) CS Tg expression stained to detect surface expression of CD23, IgD, and IgM. (C) Plots represent CD19+ splenocytes excluding MZ B cells from mice in A stained for IgM and IgD expression. Gates identify FO-II and -I subsets. (E) Plots represent peritoneal CD19+ B cells from allelic series mice with or without CS Tg expression stained with CD5 and CD23 to identify B1a (CD5hiCD23neg) and B2 (CD23hiCD5neg) subsets. (F) Plots represent CD19+ splenocytes from IgHEL Tg mice with either +/− or +/+ endogenous CD45 expression in the presence or absence of a single copy of the CS Tg stained with CD21 and CD23 to identify MZ B cells (CD21hi CD23lo). Data are representative of at least three independent experiments.