In vitro O-glycosylation analysis of known ADAM substrates. (A) Peptides of 20–28 residues covering published ADAM cleavage sites. O-glycosylated residues are indicated by GalNAc (square). (B) In vitro O-glycosylation with GalNAc-T1, -T2, -T3, -T4, -T5, -T11, -T12, -T13, -T14, and -T16. The number of incorporated sites is shown in parenthesis.