Fig. S2.

Quantification of Western blot ratios of full length and cleaved fragments of the TNF-α reporter construct. (A) Three independent biological replicates were conducted. Each of these assaying the cleavage pattern of a TNF-α reporter construct in a panel of glycoengineered HepG2 cells. Westerns blots were performed as described, and bands were analyzed using image processing program “ImageJ”. We find that the two GalNAc-T2 KO clones show a significant increase in cleavage compared with wild type, GalNAc-T1 KO, and two individual GalNAc-T2 rescue clones. Statistical analysis was performed using Student’s t test. Error bars show the SD. (B) Plasma levels (pg/mL) of IL6Ra with or without LPS induction in WT and GalNAc-T2 KO mice. Statistical analyses were performed using the Student’s t test. Error bars show SDs. (C) Hepatic expression in arbitrary units of Galnt2 and TNF-α in WT and GalNAc-T2 KO mice. Statistical analyses were performed using the Student’s t test. Error bars show the SD.