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. 2015 Nov 30;10(11):e0143892. doi: 10.1371/journal.pone.0143892

Fig 5. Characterization of the interaction of three TFs with the CmDFR promoter by yeast one-hybrid assays.

Fig 5

P53 and its promoter supplied with the kit were used as a positive control to verify the stability of these assays. The auto-activity of CmDFR promoter bait stain was tested on SD media lacking Ura in presence of AbA. CmbHLH1, CmbHLH2 and CmMYB6 were fused into pGADT7 (AD) and transformed separately into the bait stain. The binding was screened on SD media lacking Leu in presence of AbA175.