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. 2015 Nov 30;10(11):e0143892. doi: 10.1371/journal.pone.0143892

Fig 6. Protein-protein interactions between CmbHLHs and CmMYB6 studied by yeast two-hybrid assay.

Fig 6

The yeast strain was co-transformed with the indicated combinations of CmbHLH1 or CmbHLH2 fused into pGBKT7 (BD) and CmMYB6 fused into pGADT7 (AD). BD-p53 and AD-T were used as positive controls, while BD-Lam and AD-T were used as negative controls. Protein-protein interactions were detected on DDO (SD media lacking Leu and Trp) and QDO/X/A (SD media lacking Leu, Trp, His and Ade, with AbA and X-α-gal) media.