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. 2015 Oct 26;21(12):917–929. doi: 10.1093/molehr/gav058

Figure 4.

Figure 4

Effect of TLR3, RIG-I activation on HSV-2 replication. (A and B) MTT assay of End1/E6E7 cells transfected with poly I:C or 5′ppp dsRNA. End1/E6E7 cells were transfected with poly I:C (A) or 5′ppp dsRNA (B) at the concentrations indicated at the bottom of the figure. Cells viability was assessed by MTT assay 72 h post transfection. The results are mean ± SD of triplicate cultures, representative of three experiments. (C and E) End1/E6E7 cells were transfected with poly I:C at the concentrations indicated at the bottom of the figure for 24 h prior to HSV-2 infection (MOI = 0.001) (C) or transfected with poly I:C at 24 h post-infection (E). (D and F) End1/E6E7 cells were transfected with 5′ppp dsRNA at concentrations indicated at the bottom of the figure for 24 h prior to HSV-2 infection (MOI = 0.001) (D) or transfected with 5′ppp dsRNA at 24 h post-infection (F). Genomic DNA extracted from the cell cultures at 72 h post-infection was subjected to the real-time PCR for HSV-2 quantification. The data are expressed as HSV-2 gD gene levels relative (%) to the control (without poly I:C treatment, which is defined as 100). The results are mean ± SD of triplicate cultures, representing three independent experiments (**P < 0.01).