Fig 5. Selective enrichment of neonatal atrial and ventricular cardiomyocytes.

(A) P2 hearts were dissociated, depleted from cardiac non-myocytes and then sorted into ITGA6^low (PL) and ITGA6^high (PH) cells. (B) Density plots, distribution of cells before and after sorting. Dot plots, staining of the fractions for α-actinin and MLC-2a (labeled with APC anti-mouse IgG1 or IgG2ab). (C) Statistical analysis of α-actinin and MLC-2a before and after sorting. Data are expressed as mean ± SD, n = 3. t-test for paired samples with ** p ≤ 0.01 vs. before sorting.