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. Author manuscript; available in PMC: 2015 Nov 30.
Published in final edited form as: J Syst Integr Neurosci. 2015 Sep 28;1(1):20–28. doi: 10.15761/jsin.1000105

Figure 1. Adoptive transfer of CD4+ or CD8+ T cells to splenectomized male or female mice does not exacerbate infarct volume induced by focal ischemia.

Figure 1

Male and female mice were subject to splenectomy two weeks prior to transient MCAO (60 min). Mice were injected with CD4+ or CD8+ T cells 24 hours prior to MCAO. Brains were harvested 96 h after MCAO. (A) Infarct volumes measured as percentage of corrected contralateral structure for male mice injected with saline vehicle (n=10), 12 million CD4+ T cells (n=12), or 8 million CD8+ T cells (n=14) by intravenous transfer 24 h before MCAO. (B) Representative brain slices for male mice stained with TTC. Values represent mean numbers (± SEM) of splenectomized males per group analyzed using a one-way ANOVA. (C) Infarct volumes measured as percentage of corrected contralateral structure for female mice injected with saline vehicle (n=12), 12 million CD4+ T cells (n=11), or 8 million CD8+ T cells (n=11) by intravenous transfer 24 h before MCAO. (B) Representative brain slices for female mice stained with TTC. Values represent mean numbers (± SEM) of splenectomized females per group analyzed using a one-way ANOVA.