Fig 3. Halfmer formation of nacΔssbΔ knockout cells can be prevented by expression of αβ-NAC.

a-i) Polysome profiles derived from wild type (wt) or mutant yeast cells. Absorbance traces at 254 nm are shown. Cells were grown to an optical density (OD₆₀₀) of 0.8 in SD-Ura medium. 10 A₂₆₀ units of lysates of indicated cells were loaded onto 15–45% linear sucrose gradients to isolate ribosomal fractions (40S, 60S, 80S and polysomes) as indicated by centrifugation and subsequent fractionation. Polysome profiles show: a) wt + empty vector (ev), b) ssbΔ cells + ev, c-i) nacΔssbΔ cells + ev (c), + β-NAC (d), β’-NAC (e), α ^ΔUBA β-NAC (f), αβ-NAC (g) + αβ’-NAC (h) and αβ ^RRK/AAA -NAC (i) The profiles are representative for three independent experiments.