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Immunofluorescence staining of vinculin (green) and F-actin (red) in human Saos-2 osteoblasts.
Notes: 10 V (A), 20 V (B), and 30 V (C) nanotubes, on control Ti_C (D), and on glass coverslips (E) on day 3. Cell nuclei (blue) were counterstained with Hoechst 33258. Leica SPE confocal microscope, objective 63×, zoom 2×, scale 25 μm.
