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Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips.
Notes: Absorbance of collagen type I (A), ALP (B), osteopontin (C), osteocalcin (D), and calcium content (E). Measured by enzyme-linked immunosorbent assay (A–D) and by Alizarin staining (E) on day 7. Data expressed as mean ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
