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. 2015 Nov 24;9:6225–6233. doi: 10.2147/DDDT.S91968

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© 2015 Donejko et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Western immunoblot analysis for p65 NF-κB (A) protein and β-actin (B) in subconfluent human skin fibroblasts (control), cells treated with or without HA for 24 hours. The intensity of the bands staining was quantified by densitometry analysis.

Notes: Student’s t-test: **P<0.01. 1, control; 2, ethanol 25 mM; 3, ethanol 50 mM; 4, ethanol 100 mM; 5, HA; 6, ethanol 25 mM and HA; 7, ethanol 50 mM and HA; 8, ethanol 100 mM and HA. Samples used for electrophoresis consisted of 20 µg protein of pooled cell extracts.

Abbreviations: AU, arbitrary units; HA, hyaluronic acid.