Figure 2.

Nano-PSO as an α-EAE agent.

Notes: Mice were induced for EAE and treated from day 1 of the induction either with PSO or with Nano-PSO. (A) Designated EAE-induced groups were fed either with normal mouse chow (untreated group; n=10) or with chow enriched with PSO at the concentration in which 3 g (daily intake) comprises the levels designated in the figure insert: 100 (n=10) or 300 μL (n=8) PSO. P<0.05 for all PSO-treated groups versus the untreated group. (B) Designated EAE-induced groups were either left untreated or treated (by gavage) with 150 μL solution comprising 0.2, 0.8 (n=6), or 10 μL (n=7) PSO in the form of Nano-PSO. P<0.05 for 0.8 and 10 μL PSO-treated group versus the untreated group.

Abbreviations: PSO, pomegranate seed oil; EAE, experimental autoimmune encephalomyelitis.