Figure 6.

Pathological markers of EAE in Nano-PSO treated and untreated mice.

Notes: Nano-PSO treated and untreated mice were sacrificed 3 weeks after induction of EAE, and their formalin-fixed, paraffin-embedded brain sections as well as those of age-matched naïve mice (C and J) were stained by mAb EO6 (A–D and F), H&E (E and G), and LFB/PAS (brains and spinal cords) (H–J). (D) and (F) represent an enlargement of the squares in (A) and (B); (E) and (G) are serial sections of (D) and (F), respectively. Arrows in (D–G) indicate immune infiltrates. Arrows in (H) represent demyelinated areas.

Abbreviations: EAE, experimental autoimmune encephalomyelitis; PSO, pomegranate seed oil; mAb, monoclonal antibody; H&E, hematoxylin and eosin; LFB, Luxol fast blue; PAS, periodic acid Schiff.