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. Author manuscript; available in PMC: 2015 Nov 30.
Published in final edited form as: J Biol Chem. 2006 Mar 2;281(17):11627–11636. doi: 10.1074/jbc.M600897200

FIGURE 6. p190RhoGAP is a substrate of PTP-PEST.

FIGURE 6

A, lysates of HeLa cells pretreated with pervanadate were incubated with fusion proteins corresponding to PTP-PEST trapping mutants, C231S (CS), or D199A (DA) or wild type (WT) PTP-PEST. LYS, lysate. Western blotting (WB) shows that p190RhoGAP is present in the pull down using the trapping mutant (CS) but not wild type fusion protein. B, tyrosine phosphorylation of p190RhoGAP is enhanced in PTP-PEST −/− cells. In addition, the association of p190RhoGAP and p120RasGAP is increased in PTP-PEST −/− cells. IP, immunoprecipitate.

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