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. 2015 Dec 1;6:1332. doi: 10.3389/fmicb.2015.01332

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Copyright © 2015 Li, Wang, Liu, Wei, Lin, Li, Li, Dong, Cui, Hu, Li, Ma, Zhao, Liu and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity of EBOV NP detection by RT-LAMP. (A) Turbidity was monitored and recorded every 6 s by a Loopamp real-time turbidimeter at 650 nm. (B) Visual detection using a calcein fluorescent detection reagent. Lane 1, positive control (artificial EBOV RNA); lane 2, negative control (double-distilled water); lane 3, Sudan EBOV (artificial Sudan EBOV RNA); lane 4, MARV (artificial MARV RNA); lane 5, SARS coronavirus; lane 6, H7N9; lane 7, H1N1; lane 8, H2N3; lanes 9–12, human parainfluenza viruses (PIV) 1, 2, 3, and 4; lanes 13–15, adenoviruses (ADV; serotypes 3, 5, and 55); lanes 16 and 17, respiratory syncytial virus infection, RSVA, RSVB; lane 18, MERS RNA; lane 19, human metapneumovirus, HMPV; lane 20, bocavirus, BoV; lanes 21–24, human coronavirus, HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1; lane 25, Legionella pneumophila 9135; lane 26, Mycobacterium tuberculosis 005; and lane 27, Haemophilus influenza ATCC 49247.