FIGURE 3.

Effect of ELP on the accumulation levels of ER-GFP, sec-RFP and ER-TG2 and vac-TG2. The same amount of total extract was loaded on the gel as can be observed by the amount of RLS stained with Coomassie Brilliant Blue R-250. The immunoblot was developed with anti-GFP, anti-RFP, and anti-TG2 mAb 2G3, for ER-GFP, sec-RFP and ER-TG2, and vac-TG2, respectively. The intensity of the band was quantified by using ImageJ. Three biological independent experiments were performed and each replicate was obtained using leaves from five different plants. Error bars are standard error of the mean (SEM). ^∗∗Denotes statistically significant difference by Student’s t-test (P < 0.01).