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Hypoxia sample collection and quality control. (A) RNA collection scheme. Stage-synchronized embryos were collected in duplicate at gastrula (shield, 6 h) and segmentation (8-somite, 12 h) stages for RNA extraction with or without 2 h of severe hypoxia exposure at 0.3% oxygen (blue arrows). (B) Raw data from individual samples is shown both as a log-intensity boxplot and histogram. (C) Data after Robust Multi-array Average (RMA) normalization showing alignment.
