Fig. 4.

In vivo hypoxic stress protection assay. Candidate genes were assessed for a functional role in protection from hypoxic stress through two assays, both of which were performed with targeted morphant (knockdown; red needle and red outline) and embryos injected with control morpholino oligonucleotides. For acute hypoxia, larvae were subjected to hypoxia at 1.5 dpf and evaluated for exacerbation of lethality at 5 dpf (blue bolt). For the hypoxic preconditioning assay, larvae were preconditioned through a milder hypoxia exposure at 1 dpf (yellow bolt) and re-exposed to prolonged severe hypoxia at 1.5 dpf (blue bolt).