Specifications
Organism/cell line/tissue	Human neutrophils extracted from blood samples
Sex	22 males and 2 females
Sequencer or array type	Agilent DNA SurePrint G3 Human Gene Expression 8x60k v2 Microarray Kit
Data format	normalized data: TSV table with an ID column and a log_ratio column (log2 Cy3/Cy5 ratio)
Experimental factors	Samples are from 4 groups: Adults and elderly with sepsis and adults and elderly healthy controls
Experimental features	Neutrophils were collected from blood samples using a Ficoll gradient. Total RNA was isolated using TRIzol and used to generate Cy3 labeled targets (Agilent Low Input Quick Amp Labeling Kit — cat # 5190-2306). A common reference (Universal Human Reference RNA — Agilent cat # 740000) was labeled with Cy5 dye. Equimolar amounts of Cy3/Cy5 labeled targets were combined and hybridized to microarrays.
Consent	Not applicable
Sample source location	Blood samples were collected from septic patients and healthy donors at the Hospital das Clínicas Intensive Care Unit, University of Sao Paulo, Brazil.