Specifications | |
---|---|
Organism/cell line/tissue | Mus musculus/mouse embryonic stem cells (iGPA-Myo7a:mVenus line) |
Sex | N/A |
Sequencer or array type | Affymetrix mouse gene 2.1 ST Array |
Data format | Raw or analyzed |
Experimental factors | iHCs or iHCs treated with retinoic acid at day 8 and day 12 vs untreated iGPA-Myo7a:mVenus-derived Embryoid Bodies at day 8 and day 12 |
Experimental features | iGPA-Myo7a:mVenus-derived Embryoid Bodies treated with doxycycline (to activate GPA expression) or treated with doxycycline + retinoic acid during 4 or 8 days were sorted by FACS to purify iHCs (Myo7a:Venus positive cells). The iHCs were compared to untreated iGPA-Myo7a:mVenus-derived Embryoid Bodies at the corresponding time point to determine the transcriptional signature of iHCs. |
Consent | N/A |
Sample source location | Lisbon, Portugal |