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. 2015 Aug 18;6:77–80. doi: 10.1016/j.gdata.2015.08.017
Specifications
Organism/cell line/tissue Mus musculus/mouse embryonic stem cells (iGPA-Myo7a:mVenus line)
Sex N/A
Sequencer or array type Affymetrix mouse gene 2.1 ST Array
Data format Raw or analyzed
Experimental factors iHCs or iHCs treated with retinoic acid at day 8 and day 12 vs untreated iGPA-Myo7a:mVenus-derived Embryoid Bodies at day 8 and day 12
Experimental features iGPA-Myo7a:mVenus-derived Embryoid Bodies treated with doxycycline (to activate GPA expression) or treated with doxycycline + retinoic acid during 4 or 8 days were sorted by FACS to purify iHCs (Myo7a:Venus positive cells). The iHCs were compared to untreated iGPA-Myo7a:mVenus-derived Embryoid Bodies at the corresponding time point to determine the transcriptional signature of iHCs.
Consent N/A
Sample source location Lisbon, Portugal